Expressing:Article Title: Presynaptic α 2 δs specify synaptic gain, not synaptogenesis, in the mammalian brain.
Article Snippet: .. HdAd vectors expressing Cre, Mito-V5-APEX2, and EGFP were made from a previously constructed pdelta 28E4 plasmid that contained Mito-V5-APEX2 and EGFP.31 Briefly, MitoV5-APEX,78 which was a gift from Alice Ting (Addgene, plasmid #72480; http://n2t.net/addgene:72480; RRID: Addgene_72480), was cloned into the synapsin expression cassette using the EcoRI and NotI sites.79 This Mito-V5-APEX2 expression cassette was then cloned into a modified version of pdelta 28E4 using the AscI site, then further modified to contain separate syn EGFP and syn Cre expression cassettes to create a Mito-V5-APEX-Cre-EGFP construct.26 HdAd vectors containing only Cre and EGFP or myr- EGFP were created as above, but with the syn Cre expression cassette inserted into the AscI site. ..
Construct:Article Title: Presynaptic α 2 δs specify synaptic gain, not synaptogenesis, in the mammalian brain.
Article Snippet: .. HdAd vectors expressing Cre, Mito-V5-APEX2, and EGFP were made from a previously constructed pdelta 28E4 plasmid that contained Mito-V5-APEX2 and EGFP.31 Briefly, MitoV5-APEX,78 which was a gift from Alice Ting (Addgene, plasmid #72480; http://n2t.net/addgene:72480; RRID: Addgene_72480), was cloned into the synapsin expression cassette using the EcoRI and NotI sites.79 This Mito-V5-APEX2 expression cassette was then cloned into a modified version of pdelta 28E4 using the AscI site, then further modified to contain separate syn EGFP and syn Cre expression cassettes to create a Mito-V5-APEX-Cre-EGFP construct.26 HdAd vectors containing only Cre and EGFP or myr- EGFP were created as above, but with the syn Cre expression cassette inserted into the AscI site. ..
Plasmid Preparation:Article Title: Presynaptic α 2 δs specify synaptic gain, not synaptogenesis, in the mammalian brain.
Article Snippet: .. HdAd vectors expressing Cre, Mito-V5-APEX2, and EGFP were made from a previously constructed pdelta 28E4 plasmid that contained Mito-V5-APEX2 and EGFP.31 Briefly, MitoV5-APEX,78 which was a gift from Alice Ting (Addgene, plasmid #72480; http://n2t.net/addgene:72480; RRID: Addgene_72480), was cloned into the synapsin expression cassette using the EcoRI and NotI sites.79 This Mito-V5-APEX2 expression cassette was then cloned into a modified version of pdelta 28E4 using the AscI site, then further modified to contain separate syn EGFP and syn Cre expression cassettes to create a Mito-V5-APEX-Cre-EGFP construct.26 HdAd vectors containing only Cre and EGFP or myr- EGFP were created as above, but with the syn Cre expression cassette inserted into the AscI site. ..
Clone Assay:Article Title: Presynaptic α 2 δs specify synaptic gain, not synaptogenesis, in the mammalian brain.
Article Snippet: .. HdAd vectors expressing Cre, Mito-V5-APEX2, and EGFP were made from a previously constructed pdelta 28E4 plasmid that contained Mito-V5-APEX2 and EGFP.31 Briefly, MitoV5-APEX,78 which was a gift from Alice Ting (Addgene, plasmid #72480; http://n2t.net/addgene:72480; RRID: Addgene_72480), was cloned into the synapsin expression cassette using the EcoRI and NotI sites.79 This Mito-V5-APEX2 expression cassette was then cloned into a modified version of pdelta 28E4 using the AscI site, then further modified to contain separate syn EGFP and syn Cre expression cassettes to create a Mito-V5-APEX-Cre-EGFP construct.26 HdAd vectors containing only Cre and EGFP or myr- EGFP were created as above, but with the syn Cre expression cassette inserted into the AscI site. ..
Modification:Article Title: Presynaptic α 2 δs specify synaptic gain, not synaptogenesis, in the mammalian brain.
Article Snippet: .. HdAd vectors expressing Cre, Mito-V5-APEX2, and EGFP were made from a previously constructed pdelta 28E4 plasmid that contained Mito-V5-APEX2 and EGFP.31 Briefly, MitoV5-APEX,78 which was a gift from Alice Ting (Addgene, plasmid #72480; http://n2t.net/addgene:72480; RRID: Addgene_72480), was cloned into the synapsin expression cassette using the EcoRI and NotI sites.79 This Mito-V5-APEX2 expression cassette was then cloned into a modified version of pdelta 28E4 using the AscI site, then further modified to contain separate syn EGFP and syn Cre expression cassettes to create a Mito-V5-APEX-Cre-EGFP construct.26 HdAd vectors containing only Cre and EGFP or myr- EGFP were created as above, but with the syn Cre expression cassette inserted into the AscI site. ..
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